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Spontaneous IgA Synthesis by Blood Mononuclear Cells in Alcoholic Liver Disease
Author(s) -
WIEL A.,
SEIFERT W. F.,
LINDEN J. A.,
GMELIGMEYLING F. H. J.,
KATER L.,
SGHUURMAN H. J.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb01062.x
Subject(s) - peripheral blood mononuclear cell , alcoholic liver disease , antibody , immunoglobulin a , immunology , lysis , liver disease , biology , immunoglobulin g , chemistry , medicine , in vitro , biochemistry , cirrhosis
Changes in the concentration and composition of serum immunoglobulin A (IgA) and deposits of IgA in tissues are well‐known characteristics of alcoholic liver disease. We invesitigate whether these changes also accompany IgA synthesis by peripheral bloos mononuclear cells, (MNC), by counting immunoglobuliln‐producing cells using a solid‐phase enzymatic ‘spot’ test, and by analysis of immunoglobulin content in lysed cells with culture supernatant using conventional enzymatic methods. Patients with alcoholic liver disease exhibited a significantly higher number of spontaneously IgA‐producing cells than did normal healthy controls (1.7 × 10 6 cells/1 blood and 0.5 × 10 6 cells/1 blood, respectivly, P<0.01). The IgA content of MNC directly after isolation was also higher (38 and 13 ng/10 6 MNC, respectively, P<0.01), as was the IgA production during an unstimulated 6‐day culture period (520 and 95 ng/10 6 MNC put into culture, respectively, P<0.001).The spontaneously IgA‐producing cells assessed directly after isolation of mononuclear cells correlated with the IgA production during an unstimulated culture (P<0.01). We conclude that in alcoholic liver disease, B Iymphocytes circulate which may have been activated in vivio.