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Vaccination‐Induced Circulation of Human B Cells Secreting Type‐Specific Antibodies against Pneumococcal Polysaccharides
Author(s) -
HEILMANN C.,
HENRICHSEN J.,
PEDERSEN F.K.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb01047.x
Subject(s) - antibody , immunization , antigen , immunology , vaccination , peripheral blood mononuclear cell , immune system , b cell , pneumococcal vaccine , biology , microbiology and biotechnology , streptococcus pneumoniae , virology , medicine , in vitro , biochemistry , antibiotics
Indirect plaque‐forming cell assays detecting B cells secreting antibodies against capsular pneumococcal polysaccharide (PPS) antigens are described. In healthy adult volunteers the total number of B cells secreting IgM antibodies against the antigens in a polyvalent PPS vaccine reached a maximum in the blood 6 days after in vivo immunization (mean: 552/10 6 mononuclear cells), whereas the highest concentration of IgG and IgA antibody‐secreting cells (SC) were detected 7 days after immunization (means: 628 and 1691/10 6 ). B cells secreting antibodies to PPS type 3 (PPS3), PPS8, PPS18C and C‐polysaccharide (CPS)—a cell wall antigen common to all pneumococci—constituted 9%, 16%, 6% and 5% (means) of the total number of antibody SC respectively. While the majority of the anti‐PPS‐SC secreted IgA antobodies, the anti‐CPS‐SC almost exclusively secreted IgG. Pre‐vaccination concentrations of anti‐PPS were generally low in contrast to antibodies against CPS, which were present in high concentrations in all individuals. The discrepancy in the Ig class of the antibody SC is probably related to the difference in the pre‐vaccination immunity against PPS and CPS.