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The Cytotoxic T‐Cell Response to la Antigens: Lack of Correlation between the Level of Specific Cytotoxicity Obtained in Primary Mixed Lymphocyte Reaction and the Frequency of Specific Cytotoxic T Lymphocyte
Author(s) -
BERETTA A.,
DEMOYEN P. LANGLADE,
LARSSON E.L.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02183.x
Subject(s) - ctl* , cytotoxic t cell , cytotoxicity , antigen , t lymphocyte , immunology , mixed lymphocyte reaction , population , lymphocyte , t cell , microbiology and biotechnology , biology , immune system , in vitro , medicine , biochemistry , environmental health
We have investigated the cytotoxic response to allogeneic I‐E and I‐A antigens in bulk culture and limiting dilution experiments. A high degree of specific killing could be generated from unprimed T cells of I‐E‐negative strains upon stimulation with cells expressing I‐E. In such conditions, cytotoxic T lymphocytes (CTL) were generated in the absence of a specific proliferative response. The frequency determinations by means of limiting dilution experiments showed that I‐E‐specific CTL precursors were much less frequent than the K. and D specific precursors. The results suggest the existence of a population of I‐E‐specific CTL that failed to grow under conditions that allowed the growth of class I‐specific CTL.