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Flow Cytometric Analysis of Lymphocyte Subsets in the Bronchoalveolar Lavage Fluid and Peripheral Blood of Healthy Volunteers
Author(s) -
YAMADA M.,
TAMURA N.,
SHIRAI T.,
KIRA S.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02171.x
Subject(s) - bronchoalveolar lavage , cd8 , cytotoxic t cell , immunology , cd16 , lymphocyte , immune system , flow cytometry , t lymphocyte , peripheral blood , medicine , biology , lung , cd3 , in vitro , biochemistry
The lymphocyte subsets in the bronchoalveolar lavage fluid (BALF) and the peripheral blood of 25 healthy volunteers were examined by analysis with a fluorescence‐activated cell sorter. Comparison of the lymphocyte subsets in the BALF with those of the peripheral blood revealed much higher values for the ratios of each Leu. 3A + (CD4). Leu. 3 + 8 − and Leu 2 + 15 − cells, while the ratios of Leu 1 + (CD5). Leu 2a + (CD8). Leu 7 + . Leu 8 + . Leu 10 + . Leu 11a + (CD16), Leu 12 + and Leu 2 + 15 + cells were low in the BALF. The above results indicate that the lymphocyte subsets in the BALF from healthy individuals are mainly composed of cells with surface phenotypes of helper T cells and cytotoxic T cells with virtual absence of cells carrying suppressor T and NK cell phenotypes. and with low B cell ratio. Therefore, it is assumed that the local immune mechanism of the lung is different from that of the peripheral blood.