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Human Epidermal Langerhans Cells and Peripheral Blood Monocytes
Author(s) -
RÄSÄNEN L.,
LEHTO M.,
JANSÉN C.,
REUNALA T.,
LEINIKKI P.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02164.x
Subject(s) - langerhans cell , monocyte , immunology , suction blister , cell , peripheral blood mononuclear cell , antigen presentation , chemistry , t cell , antigen , biology , immune system , in vitro , biochemistry , anatomy
We compared the functional capacities of human epidermal Langerhans cells (LC) and peripheral blood monocytes. Epidermal sheets were obtained by a suction blister device. After enzymatic treatmenl LC were enriched by attaching them to IgG‐located erythrocyte monolayers. On a per cell basis, LC were several times more efficient accessory cells than monocytes in augmenting nickel‐ and tuberculin (PPD)‐induced T‐cell proliferation. In mixed cell cultures LC stimulated both autologous and allogeneic T cells, whereas monocytes stimulated only allogeneic cells In addition. LC were significantly more potent allogeneic stimulators than monocytes. Although monocytes were weaker accessory cells and allogeneic stimulators than LC, they induced higher interleukin 1 (IL‐1) activities than LC‐enriched or LC‐depleted cells. These results indicate that there are functional differences between LC and monocytes and that antigen presentation and mediator secretion are not correlated.