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Monoclonal Antibodies Specific for Sendai Virus I. Production and Characterization of Monoclonal Antibodies
Author(s) -
FENNER M.,
BINZ H.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02102.x
Subject(s) - epitope , monoclonal antibody , virology , sendai virus , antibody , biology , microbiology and biotechnology , glycoprotein , virus , immune system , immunology
Twelve monoclonal antibodies specific for Sendai virus were prepared by fusing immune LOU rat spleen cells with Y3 or FO myeloma cells. Six antibodies bound the viral glycoprotein HN, and six the viral protein F. Among the six HN‐specific monoclonal antibodies, five reacted with the very same epitope and inhibited viral haemagglutination. Two antibodies against the F protein recognized the same epitope, but all the others reacted with different epitopes. All monoclonals were characterized with regard to specificity, biological function, epitope recognition, isotypes, and pI.