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Insignificant Immune Phagocytosis Inhibition and Cytotoxicity by the Murine Monoclonal HLA‐DP Antibody B7/21 Tested on Human Monocytes and Macrophages
Author(s) -
NEPPERT J.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02100.x
Subject(s) - monoclonal antibody , phagocytosis , cytotoxicity , antibody dependent cell mediated cytotoxicity , flow cytometry , immune system , macrophage , antigen , immunology , antibody , monocyte , biology , complement dependent cytotoxicity , human leukocyte antigen , microbiology and biotechnology , chemistry , in vitro , biochemistry
On the basis of fluorescence flow cytometry data with the monoclonal antibody (MoAb) B7/21, and of the differing cell sizes, the HLA‐DP antigen density on monocytes is estimated to be 25% of that on B lymphocytes. The remote positions of the antigens are assumed to be responsible for the markedly reduced inhibitory capacity of the MoAb B7/21 in immune phagocytosis by monocytes and for the absence of complement‐dependent cytotoxicity of this MoAb to monocytes. The findings from experiments on peritoneal macrophages are comparable. Therefore HLA‐DP may not be important for the functioning of the monocytes and macrophages.