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Characterization of a Monoclonal Antibody Recognizing a Monomorphic Determinant of the Alpha Chain of Class II DQ Antigens
Author(s) -
FERMAND J.P.,
CHEVALIER A.,
BROUET J.C.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02099.x
Subject(s) - epitope , monoclonal antibody , immunoprecipitation , microbiology and biotechnology , antigen , immunofluorescence , blot , antibody , alpha chain , human leukocyte antigen , biology , chemistry , immunology , gene , genetics
We have characterized a monoclonal antibody (L2) specific for a determinant of Human DQ class II antigens. After immunoprecipitation and immunofluorescence analysis performed on a panel of DR homozygous cell lines and HLA deleted mutants, it behaves like a monomorphic anti‐DQ reagent and does not react with the products of the various DR alleles. On immuno‐blotting, it reacts with an epitope of the DQ a chain.