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Participation of Interleukins in Synergistic Effect of Bu‐WSA on Concanavalin A‐Induced DNA Synthesis in Mouse Splenic Lymphocytes
Author(s) -
NITTA T.,
KONNOEJIRI H.,
NEMOTO K.,
OKUMURA S.,
OZAWA A,
NAKANO M.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02097.x
Subject(s) - concanavalin a , dna synthesis , spleen , population , biology , microbiology and biotechnology , interleukin 2 , stimulation , lymphocyte , thymidine , cell culture , immunology , cytokine , biochemistry , dna , in vitro , endocrinology , medicine , genetics , environmental health
Butanol‐extracted water‐soluble adjuvant (Bu‐WSA) obtained from Bacterionema matruchotti was mitogenic to murine splenic B lymphocytes, but not T lymphocytes. When murine splenic cells were cultured in the presence of Bu‐WSA and concanavalin A (Con A) together. [ 3 H|thymidine uptake of the culture cells synergically increased. The mechanism of the synergy of Con A and Bu‐WSA and the participation of interleukin (IL) 1 and 2 in the synergy were studied. The proliferation cells in the synergy were I yt‐1 − 23 − lymphocytes.’la‐positive accessory cells were required for the response. When separated cell populations and Marbrook‐type culture vessels were used, a mixed cell population of T lymphocytes and B lymphocytes or macrophages (Mφ) produced some active factor(s) after co‐stimulation by Con A and Bu‐WSA, and the factors enhanced DNA synthesis of another Con A‐activatcd T lymphocyte population. Supernatants obtained from the spleen cell cultures or the mixed cell cultures with T lymphocytes and Mφ in the presence of Con A and Bu‐WSA contained greater amounts of IL‐1 and IL‐2 than those from cultures containing Con A or Bu‐WSA alone. An addition of exogenous IL‐1 or IL‐2 to spleen cell cultures with Con A resulted in a proliferative response like that obtained through co‐stimulation by Con A and Bu‐WSA. These results suggest that the synergistic effect of Con A and Bu‐WSA on the proliferative response in murine splenic cells is sustained by the enhancement of production of these T‐lymphocyte growth factors.