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Development of a Model System for Analysing Graft‐versus‐Host‐Mediated Immune Suppression
Author(s) -
DEMOYEN P. LANGLADE,
LARSSON E.L.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02077.x
Subject(s) - immune system , host (biology) , immunology , biology , genetics
F 1 , hybrid mice injected with parental T lymphocytes undergo a graft‐versus‐host (GvH) reaction, one of the consequences being a highly depressed cytotoxic T‐lymphocyte (CTL) potential against alloantigens and modified self antigens. In the present study we demonstrate that concanavalin A (Con A) can he used for analysing such GvH‐mediated immune suppression. Thus, both alloantigen‐ and Con A‐induced responses were reduced by approximately 80% in F 1 , suppressed (F 1 s) animals as compared to F 1 control (F 1 c) mice, Although interleukin 2 (IL‐2) production was found to be reduced by approximately 50% it did not account for the reduced CTL response in F 1 s mice. The addition of IL‐2 to Con A‐stimulated F 1 s spleen cell cultures did not reconstitute the response. The results suggest that the suppressive mechanism operates by preventing a large fraction of Lyt‐2 + CTL precursors from acquiring IL‐2 reactivity. However, a small fraction of CTL precursors, escaped the suppression and differentiated into effector CTL.