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Comparison of Hapten‐Specific Cytotoxic and Suppressor T Cells by a Split Culture Approach
Author(s) -
ANDRIGHETTO G.,
BENATO B.,
LOPATTA D.,
ZÖLLER M.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb02063.x
Subject(s) - cytotoxic t cell , hapten , in vivo , in vitro , microbiology and biotechnology , chemistry , spleen , t cell , cytotoxicity , biology , immunology , antibody , immune system , biochemistry
The protocols for the induction of hapten‐specific suppressor T cells (T s ) and hapten‐specific cytotoxic T cells (T c ) are essentially the same. Hence, to exclude or support the possibility of an apparent suppression of B‐cell responsiveness by elimination of B cells due to cytotoxic cells, limiting dilution cultures were concomitantly tested for suppression of a primary B‐cell response against trinitrophenol (TNP), and cytotoxic activity towards a TNP‐haptenized anti‐TNP IgM hybridoma. When compared with the spleen cells (SC) of untreated Balb/c mice, the frequency of T c was found to be increased after in vivo or in vitro induction of TNP‐specific T s (via intravenous injection of TNP‐haptenized tympocytes or cocultivation of SC with haptenized lymphocytes). Despite this synchronous increase of hapten‐specific T s and T c , the number of wells displaying both cytotoxic and suppressive activity did not exceed the number of wells that were expected to contain T s and T c . Hence, in the system described, hapten‐specific T c did not lyse anti‐hapten antibody‐producing B cells to any measurable extent, and suppression of a B‐cell response by in vivo or in vitro‐induced T s was independent of cytotoxic T‐cell activity.