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Features of Haemocyanin‐Induced Suppression in Vivo
Author(s) -
HUCHET R.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01998.x
Subject(s) - histamine , spleen , effector , in vivo , immunology , cyclophosphamide , biology , ovalbumin , antibody , chemistry , immune system , endocrinology , medicine , chemotherapy , microbiology and biotechnology
Haemocyanin (KLH, 4 mg), given intraperitoneally, induces in mice a state of unresponsiveness related only to the activity of two independent pathways of suppression. The early pathway is characterized by the depression of the anti‐trinitrophenyl response to TNP KLH within 24 h of KLH injection, and the ability of spleen cells from KLH‐treated animals to transfer unresponsiveness in normal recipients. These features of suppression are stopped after fractionation of spleen cells from KLH‐treated mice over insolubilized conjugated histamine columns. The late pathway of suppression is characterized by the nonspecificity of its effector phase, leading to a depressed anti‐FLu antibody response after a challenge with TNP KLH + FLu OVA. These features, which are cyclophosphamide‐resistant, are no longer active after passage through insolubilized conjugated histamine columns.