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Human T‐Cell Hybrids Secreting Lymphokines: Effect of Different Parent Cell Clones of the Human T‐Cell Acute Lymphoblastoid Leukaemia Line CCRF‐CEM
Author(s) -
GALLAGHER G.,
STIMSON W. H.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01996.x
Subject(s) - phytohaemagglutinin , lymphoblast , clone (java method) , biology , lymphocyte , lymphokine , concanavalin a , cell culture , t lymphocyte , hybrid , microbiology and biotechnology , peripheral blood mononuclear cell , immunology , in vitro , genetics , gene , botany
We have cloned a number of cell lines from the human T‐lymphocyte acute lymphoblastoid leukaemia (ALL) line CCFR‐CEM, and attempted to construt functionally active human T‐lymphocyte hybrids with them. Functional hybrids were generated using only one particular clone, 3H6. The activities found in She supernatant of two of these hybrids, DB1G7 and DH2DI0, are described. Supernatant from DB1G7 was found to suppress strongly the migration of normal human peripheral blood mononuclear cells, while that from DB2D10 was shown to inhibit the proliferate response of human T lymphocytes to both phylohaemagglulinin and concanuvalin A. There was no cross‐reactivity between the two supernatants, confirming the usefulness of the human T‐lymphocyte hybrid technique in dissecting human T‐lymphocyte function. The successful use of 3H6 is contrasted with (he failure of another clone, 2H2, to permit the production of functional hybrids. Problems relating to the use of CCRF‐CEM and its clones as parent cell lines in the production of human T‐lymphocyte hybrids are discussed.