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Dependency of B Cells on the Presence of Adherent Cells, or Factors Derived from Them, for the Production of Autoantibodies in Vitro in the Absence of Cell Division
Author(s) -
DAENKE S.,
COX K. O.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01989.x
Subject(s) - in vitro , biology , cell culture , secretion , autoantibody , microbiology and biotechnology , cd40 , immunology , antibody , cytotoxic t cell , biochemistry , genetics
Peritoneal cells from untreated mice secrete autoantibodies after 3–4 days of in vitro culture, although the cells do not divide. Here, peritoneal cells enriched for B cells to contain 95% surface Ig‐bearing cells, did not secrete autoantibodies after 3 days of in vitro culture unless plastic‐adherent cells derived from the peritoneal cavity were cultured with the B cells. Cell‐free media, taken from peritoneal adherent cells that had been cultured for 3 days in vitro, when added at a final concentration of 50% in fresh culture medium to purified B cells, substituted for the presence of accessory cells. In contrast to cultures of unfractionated peritoneal cells, little increase in precursor frequency was detected when enriched B cells were cultured in the presence of LPS/DXS. However, the addition of adherent cells, supernatants derived from adherent cells, or cytokines produced by a T‐cell hybrid EL4, resulted in an increased precursor frequency when LPS/DXS was added to the culture medium. Three macrophage cell lines. P3788‐D1. J774. and PU‐5‐IR, when added to purified B cells, augmented the autoantibody precursor frequency detected in vitro. This is strong evidence that potentially autoreactive B cells require one or more types of accessory cells in order to differentiate into autoantibody secretors during culture in vitro. Further, the results provide indirect evidence that interleukin 1 may be a crucial molecule in the differentiation of B cells.