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The Immune Response to Pneumococcal Polysaccharide Type III is Followed by Increased Production of an Antibody Antifactor
Author(s) -
PASANEN V. J.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01972.x
Subject(s) - antibody , concanavalin a , polysaccharide , immune system , sepharose , titer , immunology , affinity chromatography , chemistry , biology , microbiology and biotechnology , biochemistry , enzyme , in vitro
The study shows that serum from pneumococcal polysaccharide type III (SSSIII)‐immunized and nonimmunized mice that has been absorbed until devoid of anti‐SSSIII antibodies inhibits anti‐SSSIII plaque‐forming cells (PFC) but not antisheep red blood PFC in antibody plaque assay. A large fraction of the inhibitions activity can be absorbed by and eluted from anti‐SSSIII‐Sepharose, but not normal mouse serum‐Sepharose. It has low or no affinity for protein A and concanavalin A The titre of the inhibitor increases, with repeated immunizations and inhibits the anti‐SSSIII PFC of rats or mice of different allotypes. On the basis of these characteristics, I classify the inhihitor as an anti‐idiotypic nonimmunoglobulin factor directed against a common determinant of anti‐SSSIII antibodies of mouse and rat, with an immuno‐regulatory function.