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Suppressor Factors Generated from Human Mononuclear Cells by Means of Purified Myeloma Proteins
Author(s) -
WANGEL A. G.,
KONTIAINEN S.,
ARVILOMMI H.,
NUUTINEN M.,
KOISTINEN J.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01967.x
Subject(s) - peripheral blood mononuclear cell , pokeweed mitogen , isotype , myeloma protein , antibody , immunology , microbiology and biotechnology , biology , immunoglobulin g , virology , chemistry , in vitro , monoclonal antibody , biochemistry
Peripheral blood mononuclear cells (PBMC) from normal human donors were cultured in Marbrook flasks in the presence of purified IgG or IgA myeloma proteins. The culture supernatants were tested for their ability to suppress pokeweed mitogen (PWM)‐ or Epstein‐Barr virus (EBV)‐driven Ig synthesis by normal PBMC, Two supernatants from PBMC cultured with IgG and one from PBMC cultured with IgA were tested and suppressed PWM‐driven Ig synthesis as measured by a reverse haemolytic plaque assay and by quantitation of the Ig secreted into the culture medium of the PWM‐driven cells. This suppression was not restricted to the Ig isotype of the ‘inducing’ myeloma protein, but was extended to IgG, IgA, and IgM, The suppressive effect could be absorbed out with human IgG.