Premium
Characterization of Two Morphologically Distinct Leu‐7 + Cell Subsets with Respect to Leu‐15 Antigen
Author(s) -
FERRARI G. C. MANARA. C.,
SCANDROGLIO R.,
ROCCHI G.,
PAGANI L.,
PANFILIS G.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01961.x
Subject(s) - antigen , biology , genetics , microbiology and biotechnology
in the present study the fine structures of Leu‐7‐Leu‐15 + and Leu‐7 + ‐Leu‐15 cell subpopulations were characterized by using an immunogold‐immunoperoxidase double labelling in electron microscopy. The densities of Leu‐15 antigenic sites on both E rosetting (Er) and non‐adherent/non‐E rosetting (non‐A/non‐Er) Leu‐15 positive cell surfaces were also evaluated by using an immunogold analysis in electron microscopy. A majority of Leu‐7 + cells co‐expressed I he Leu‐15 antigen and showed an ultrastructural pattern specific for mature natural killer (NK) cells, i.e. abundant cytoplasm with many organelles. numerous electron dense granules and irregular outline. On the other hand, a minority of Leu‐7‐ ceils did not express the Leu‐15 antigen and showed a clearly different ultrastructural feature in comparison to Leu‐7 + ‐Leu‐15 + cells T hus, the presence of the Leu‐15 antigen on Leu‐7 + cell surface corresponds to ultrastructural features specific to differentiated NK cells and may represent an expression of Leu‐7 + cell differentiation. An alternative hypothesis may he that Leu‐7 + ‐Leu‐15‐ and l.eu‐7‐Leu 15 cells represent distinct cell lineages within non‐A/non‐Er Leu‐7 + cells. Finally, the results of the present study provide proof that Leu‐7 + antigen is more frequently represented on non‐A/non‐Er Leu‐L‐15 + cells than on Er Leu‐15 + cells.