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Production of Interferon‐β by Murine T‐Cell Lines Induced by 10‐Carboxymethyl‐9‐Acridanone
Author(s) -
STORCH E.,
KIRCHNER H.,
BREHM G.,
HÜLLER K.,
MARCUCCI F.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01958.x
Subject(s) - newcastle disease , concanavalin a , biology , cell culture , beta (programming language) , microbiology and biotechnology , t cell , interferon , virus , virology , interferon gamma , cytokine , immunology , in vitro , immune system , biochemistry , genetics , computer science , programming language
Besides the established T‐cell property of producing gamma interferon (IFN‐γ), murine T cells additionally possess the ability to produce IFN‐α and IFN‐β when appropriate inducers such as 10‐carboxymethyl‐9‐acridanone (CMA) or Newcastle disease virus (NDV) are used, Inter‐leukin 2 (IL‐2)‐dependent murine T‐cell lines, but not purified resting splenic T cells, responded to CMA and NDV with production of IFN‐α, β, The IFN production by these T cells was not restricted to a special subset, since T cells expressing the Lyt 1 + 2 − and the Lyt 1 − 2 + phenotype responded to these inducers with IFN production, After prolonged passaging of the T‐cell lines in IL‐2‐containing medium, the ability to respond to CMA with production of antiviral activity was sustained longer than the ability for concanavalin A‐induced WH‐y production. Whereas the NDV‐induced T‐cell supernates contained both IFN‐α and IFN‐β, the induction with CMA resulted exclusively in the synthesis of IFN‐β by the T‐cell lines.