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Induction of Concanavalin A Dose‐dependent T‐Cell Growth Factor Production by Insertion of T‐Cell Membrane Components into the AKR Thymic Lymphoma BW 5147
Author(s) -
LINDQVIST C.,
SHAPIRO I. M.,
WIGZELL H.
Publication year - 1986
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1986.tb01949.x
Subject(s) - concanavalin a , t cell , biology , cell , cell growth , cell culture , lymphoma , microbiology and biotechnology , transplantation , major histocompatibility complex , immunology , antigen , biochemistry , medicine , immune system , genetics , in vitro
Sendai virus vesicles were used as vehicles for the insertion of various cell membranes into different ceil lines. The transplantation efficiency was measured by using FITC‐labelled concanavalin A (Con A) or monoclonal antibodies against the T‐cell marker Lyt 2 and the major histocompatibility complex product H‐2D h in a fluorescence‐activated cell sorter. Results indicate that it is possible to transplant mitogen responsiveness to certain cell types. Con A stimulation of T‐cell membrane transplanted BW 5147 showed that it is possible to induce a mitogen dose‐dependent T‐cell growth factor production. Consequently this method appears to he an attractive model for further study of the properties of the membrane structures involved in mitogen triggering of cells.