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Rabbit Anti‐Human β 2 Microglobulin Antibodies: A T‐Cell Mitogen
Author(s) -
HAMMARSTRÖM L.,
SMITH C. I. E.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01930.x
Subject(s) - beta 2 microglobulin , microbiology and biotechnology , antibody , t cell , population , beta (programming language) , biology , cell , chemistry , immunology , biochemistry , medicine , immune system , environmental health , computer science , programming language
Rabbit anti‐β 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T‐cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non‐T cells. Daudi cells (which do not express β 2 microglobulin on their cell surface) were shown to produce ‘macrophage replacing factors’ which supported anti‐β 2 ‐induced T cell division. Non‐T cells could be effectively replaced by addition of interleukin 2 containing cell supernatants, 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate (TPA), or teleocidin B. Thus, anti‐β 2 appears to selectively activate T cells. In contrast to other T cell mitogens, anti‐β 2 microglobulin antibodies did not induce secondary immunoglobulin production in B cells from peripheral blood or spleen.