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Loss and Re‐acquisition of Lytic Function by Cloned Cytotoxic T Lymphocytes: Role of Specific Antigen and Interleukin 2
Author(s) -
OCHOA A. C.,
WEE S.L.,
BACH F. H.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01929.x
Subject(s) - ctl* , lytic cycle , cytotoxic t cell , reversion , interleukin 2 , biology , antigen , receptor , t lymphocyte , cytolysis , microbiology and biotechnology , immunology , immune system , phenotype , in vitro , biochemistry , virus , gene
We have previously reported the isolation of 2 human allospecific cytotoxic T‐lymphocyte (CTL) elones that can undergo a reversible functional change from a highly lytic phase to a nonlytic quiescent phase and again to a lytic phase. The entire process was found to be regulated by recombinant interleukin 2 (rIL‐2). We have now extended these studies in 3 ways. First, we show that specific alloantigens can also function as a signal to reactivate lytic function in the reverted CTL. However, in contrast to CTL reactivated with rIL‐2, the antigen‐reactivated CTL apparently fail to undergo subsequent cell division. Second, we have also found that this reversion phenomenon is not the same for all CTL tested, as 2 other CTL clones were found that did not revert to the non‐lytie phase when cultured for up to 60 h in rIL‐2‐free medium. Third, the expression of Tac (IL‐2 receptor) was also studied throughout the process of reversion and reactivation. rIL‐2 added to the cell that had reverted to a non‐lytic phase induced, an increase in the expression of Tac receptors during the reactivation phase, to levels greater than those expressed on cells continuously cultured in rIL‐2‐supplemented medium.