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Elimination of Trinitrophenol‐Specific Antibody Response by Antigen‐Toxin Conjugates
Author(s) -
ARNDT R.,
THIESEN H.J.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01907.x
Subject(s) - ricin , antigen , keyhole limpet hemocyanin , hemocyanin , immune system , toxin , spleen , in vitro , antibody , biology , immunology , chemistry , microbiology and biotechnology , biochemistry
The aim of the experiments presented here is the selective inhibition of an antigen‐specific immune response. Antigen receptors are used as targets for conjugates of antigens and toxin to eliminate antigen‐reactive cells. The trinitrophenol (TNP)‐Specific immune response can be specifically abrogated by incubating the TNP‐keyhole limpet haemocyanin (KLH)‐primed spleen cells with TNP‐ricin or TNP‐chicken IgG‐ricin conjugates before in vitro stimulation with TNP‐KLH. The rate of elimination is dose‐dependent and related to the degree of TNP moieties bound to the toxin molecule. The specificity of the toxin conjugates is demonstrated by treating sheep erythrocyte‐primed spleen cells with TNP‐lgG‐ricin conjugates. These results may have therapeutic relevance for treating autoimmune diseases.