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Monoclonal Antibody‐Defined β 2 ‐Microglobulin‐Positive Mononuclear Cells in Acquired Immune Deficiency Syndrome
Author(s) -
GUPTA S.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01892.x
Subject(s) - beta 2 microglobulin , peripheral blood mononuclear cell , immune system , population , monoclonal antibody , aids related complex , immunology , antibody , asymptomatic , arc (geometry) , monoclonal , intensity (physics) , medicine , biology , virus , viral disease , in vitro , genetics , geometry , mathematics , environmental health , quantum mechanics , physics
In healthy heterosexuals, by means of a monoclonal antibody against β 2 ‐microglobulin (β 2 m) and fluorescence‐activated cell sorter analyser (FACS analyser), we observed two populations of β 2 + mononuclear cells (MNC); a large population with high‐intensity cells and a small population of relatively low‐intensity cells. Because elevated β 2 m levels have been shown in the sera of patients with acquired immune deficiency syndrome (AIDS), we examined the proportions of β 2 m + MNC in AIDS and AIDS‐related complex (ARC). The proportions and numbers of high‐fluorescence intensity β 2 m + MNC were significantly (P<0.05) decreased in AIDS and ARC when compared with those of asymptomatic homosexuals and healthy heterosexuals. The proportion and number of total β 2 m + MNC (both high‐ and low‐intensity β 2 m + ) in ARC were comparable to those of control groups; however, the numbers of total β 2 m + MNC were significantly (P<0.01) reduced only in the AIDS group. This relative deficiency of highintensity β 2 m + MNC appears to be due to a shift from high‐intensity β 2 m + to low‐intensity β 2 m + MNC. A linear correlation was observed between the deficiency of high‐intensity β 2 m + cells and T helper to T suppressor phenotype cell ratios.