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Detection of Cell‐Mediated Lympholysis Determinants Encoded by the I‐A and I‐E Genes on Ultraviolet‐Irradiated Spleen Cells
Author(s) -
BERETTA A.,
LARSSON E.L.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01880.x
Subject(s) - cytotoxic t cell , congenic , antigen , spleen , microbiology and biotechnology , biology , t cell , cell , immunology , gene , immune system , in vitro , biochemistry
We have analysed the effects of ultraviolet (UV) irradiation on the expression, by normal splenic cell populations, of cell‐mediated lympholysis determinants encoded by the I‐A and I‐E genes of the H‐2 complex. Cytotoxic T lymphocytes specific for class II antigens could be generated from unprimed responder mice when stimulated with UV‐treated cells from I‐A‐and/or I‐E‐congenic mice in the presence of interleukin 2‐containing medium. A cytotoxic T‐cell line specific for I‐E k antigens was obtained and maintained upon stimulation with UV‐irradiated spleen cells. Secondary la‐specific responses were obtained with UV‐irradiated stimulators in the absence of added soluble factors. The results show that the failure of UV‐irradiated cells to stimulate primary cytotoxic T‐cell responses is not due to a selective destruction of class II antigens but rather to the inactivation of a still undefined function of la‐positive cells, other than interleukin‐1 production, involved in the establishment of high‐affinity interactions between the responding T helper cells and the stimulator cells.