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Magnetic Monosized Polymer Particles for Fast and Specific Fractionation of Human Mononuclear Cells
Author(s) -
LEA T.,
VARTDAL F.,
DAVIES C.,
UGELSTAD J.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01873.x
Subject(s) - peripheral blood mononuclear cell , polymer , dispersity , chemistry , divinylbenzene , monoclonal antibody , fractionation , magnetite , styrene , chemical engineering , biophysics , materials science , chromatography , polymer chemistry , antibody , biochemistry , organic chemistry , immunology , in vitro , biology , copolymer , metallurgy , engineering
Magnetic monodisperse polymer particles were developed and the necessary conditions established to use them for both quantification and fractionation of human peripheral blood mononuclear cell populations. The particles consist of a styrene divinylbenzene core into which magnetite has been deposited by an in situ oxidation process. Thereafter the core has been coated with a hydrophilic polymer containing epoxy and hydroxyl groups. The particles have strong nonspecific binding capacity for protein and can he coaled with the appropriate antibodies by physical adsorption only. However, the hydroxyl groups on the outer polymer also make covalent coupling possible. After appropriate blocking they can be used in a rosette assay for quantification of mononuclear leukocytes previously sensitized with monoclonal antibodies. Furthermore, a suitable magnet makes it possible to deplete the cell suspension efficiently of the rosette‐forming cells. We have thoroughly investigated the functional properties of human mononuclear cells depleted of T lymphocytes by this technique. Our results show that T cells are virtually completely eliminated, as demonstrated by flow cytometry and various functional assay systems.