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Monoclonal Antibodies Recognizing a Neoantigen of Poly(C9) Detect the Human Terminal Complement Complex in Tissue and Plasma
Author(s) -
MOLLNES T. E.,
LEA T.,
HARBOE M.,
TSCHOPP J.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01870.x
Subject(s) - monoclonal antibody , antibody , complement membrane attack complex , microbiology and biotechnology , chemistry , polyclonal antibodies , ethylenediaminetetraacetic acid , complement system , monoclonal , immunohistochemistry , gel electrophoresis , antigen , biochemistry , biology , immunology , chelation , organic chemistry
The terminal complement complex (TCC), consisting of C5b, C6, C7, C8, and C9, contains neoantigens that are absent from the individual native components. Neoantigens are present both in the membrane‐bound (MAC) and the fluid‐phase (SC5b‐9) complex. The present study describes production of monoclonal antibodies against neoantigens of both forms of the TCC. A convenient screening and detection system, based mainly on enzyme‐linked immunosorbent assays, crossed immunoelectrophoresis with autoradiography, and affinity chromatography with subsequent sodium dodecyl sulphate‐polyacrylamide gel electrophoresis including immunoblotting, is described in detail. Two monoclonal antibodies were specific for a neoantigen located in the poly(C9) moiety of the TCC. One of these antibodies, MCaE11, was used for immunohistochemical detection of MAC in tissue and for quantification of the fluid‐phase TCC in ethylenediaminetetraacetic acid plasma.