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Passage of Undegraded Dietary Antigen into the Blood of Healthy Adults
Author(s) -
HUSBY S.,
JENSENIUS J. C.,
SVEHAG S.E.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01862.x
Subject(s) - ovalbumin , antigen , immune system , antibody , chemistry , gel permeation chromatography , absorption (acoustics) , enzyme , endocrinology , immunology , high performance liquid chromatography , medicine , chromatography , biochemistry , physics , organic chemistry , acoustics , polymer
The absorption into the blood of ovalbumin (OA) and beta‐lactoglobulin (BLG) was investigated in eight healthy adults. Enzyme immunoassays showed up to 10.5 ng OA/ml serum in seven out of eight individuals 2‐3 h after a test meal, whereas no BLG was demonstrated. The apparent size distribution of the absorbed OA was investigated by high‐pressure liquid gel permeation chromatography fractionalion, followed by enzyme‐linked immunosorbemt assay, which in the fractionated sera showed OA in all eight individuals. OA was found at the elution volume of intact OA and, in addition, was present in high molecular weight fractions, as part of the immune complexes. The serum concentrations of OA could not be clearly correlated to levels of serum and secretory anti‐OA antibodies. No significant alterations of the levels of circulating immune complexes could be demonstrated by two antigen‐nonspecific assays. The presented data indicate that, for certain dietary proteins, low‐grade absorption of apparently undegraded protein into the blood occurs regularly in healthy adults.