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Selective Inhibition of IgE versus β 2 ‐Microglobulin in Human U‐266 Myeloma Cell Line Treated with T‐Cell‐Derived Factors
Author(s) -
ROSSI P.,
GALLI E.,
GIDLUND M.,
SALAHUDDIN Z.,
LAAN K.,
WIGZELL H.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01857.x
Subject(s) - immunoglobulin e , beta 2 microglobulin , cell culture , concanavalin a , microbiology and biotechnology , effector , population , biology , cell , immunology , antibody , medicine , biochemistry , genetics , in vitro , environmental health
Concanavalin‐A‐activated T cells and their crude supernatant were assayed for suppressivc activity un an IgE‐producing U‐266 cell line. Delectable and comparable degrees of suppression were obtained with the co‐culture and the supernatant protocols. Separation of the effector population into T4 + and TS + subsets showed the most effective cells in the T8 + fraction. Control experiments demonstrated that the IgE down‐regulation was selective, since parallel measurement of β 2 ‐microglobulin synthesis showed no effect of T cells or T‐cell‐derived supernatants. In addition, several human T‐cell lymphoma‐leukaemia virus I‐transformed T‐cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U‐266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level, The results indicate that the T‐cell‐mycloma system is an interesting model to define belter the regulation of IgE in the human.