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Quantification of the C3d Split Products of Human Complement by a Sensitive Enzyme Linked Immunosorbent Assay
Author(s) -
MOLLNES T. E.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01851.x
Subject(s) - polyethylene glycol , chemistry , immunoelectrophoresis , chromatography , detection limit , antibody , microbiology and biotechnology , biochemistry , immunology , medicine , biology
A double‐antibody biotin‐avidin enzyme‐linked immunosorbenl assay (ELISA) for quantification of the C3d split products is described. Polyethylene glycol MXJO was used to precipitate large C3 molecules, and the C3d‐containing supernatant was used in the assay. C3d was measured in ethylenediamineietraacetic acid plasma from 40 healthy blood donors, and the normal range wa.s defined. Twenty‐two patients were tested, and 12 of these had increased levels of C3d. No correlation was observed between total C3 and C3d in these patient;., There was a close correlation between C3d measured hy ihis method and by the double‐decker rocket immunoelectrophoresis method. The C3d ELISA method is very sensitive, easy lo perform, and lime‐saving and economical compared with most C3d methods already described. A procedure lo define the lower detection limit and examine the reliabiliiy of an ELISA method in general is discussed.