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Mycoplasma Mimicry of Lymphokine Activity in T‐Cell Lines
Author(s) -
RUUTH E.,
RÅNBY M.,
FRIEDRICH B.,
PERSSON H.,
GOUSTIN A.,
LEANDERSON T.,
COUTINHO A.,
LUNDGREN E.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01849.x
Subject(s) - lymphokine , mycoplasma , biology , microbiology and biotechnology , cell culture , clone (java method) , strain (injury) , secretion , antibody , centrifugation , virology , antigen , immunology , genetics , biochemistry , gene , anatomy
During screening of T‐T hybridomas for B‐cell‐stimulating factors (BSF) we found in the conditioned medium of such a line (TUH‐U) an activity that turned out to cosediment with a contaminating strain of Mycoplasma arginini on centrifugation. Since it only affected B blasts and only to a minor extent small cells and since it did not induce immunoglobulin secretion, the mycoplasma strain seemed to mimic a BSF. M. arginini was also found in two other T‐T hybridoma producer lines but not in a clone of the EL‐4 line. Three other M. arginini isolates were not stimulatory for B cells. The presented results point towards the importance of careful control for mycoplasma contamination in established cell lines.