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Distribution of Ig Classes and IgG Subclasses among Human B Cells Activated by Nocardia opaca ‐Delipidated Cell Mitogen or by Pokeweed Mitogen
Author(s) -
THI BICHTHUY LÈ,
CIORBARUBAROT R.,
REVILLARD J.P.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01843.x
Subject(s) - pokeweed mitogen , subclass , peripheral blood mononuclear cell , biology , antibody , microbiology and biotechnology , in vitro , immunoglobulin g , cell culture , immunology , genetics
The relative proportions of cells synthesizing the three major Ig classes or one of the four IgG subclasses in cultures stimulated with pokeweed mitogen (PWM) or Nocardia ‐delipidated cell mitogen (NDCM) were investigated. In cultures of human peripheral Mood mononuclear cells (PB MNC) stimulated with PWM, the number of IgG‐comaining cells (CC) was higher than the number of IgM‐CC, and a substantial number of IgA‐CC was found. Conversely, in NDCM‐stimulated PB MNC cultures IgM‐CC outnumbered IgG‐CC and only few IgA‐CC were detected. In those cultures, the removal of T cells resulted in an increase in the number of IgM‐CC concomitant with a decrease in the number of IgG‐CC. A substantial number of cells containing simultaneously IgG or IgA in addition to IgM could be found in PWM‐stimulated cultures. These cells were virtually absent in NDCM‐stimulated cultures. The relative proportions of IgG subclass‐CC were IgG1‐CC>IgG2‐CC>IgG3‐CC≥IgG4‐CC in PWM‐stimulated and IgG2‐CC>IgG1‐CC>IgG3‐CC≥IgG4‐CC in NDCM‐stimulated cultures. The removal of T cells from NDCM‐stimulated cultures did not result in major alteration of this distribution. The role of T cells and of the genomic order of the Igh‐C genes in their phenotypic expression triggered in vitro by PBA is discussed.