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Interferon Regulation of DR Antigen Expression and Alloantigen‐Presenting Capabilities of the Promyelocytic Cell Line HL60
Author(s) -
STEEG P. S.,
SZTEIN M. B.,
MANN D. L.,
STRONG D. M.,
OPPENHEIM J. J.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01828.x
Subject(s) - lymphokine , antigen , biology , interferon , immune system , monocyte , hl60 , immunology , interferon gamma , lymphocyte , cell culture , t lymphocyte , in vitro , microbiology and biotechnology , biochemistry , genetics
Our research suggests that interferon may have an immunoregulatory role in the initiation phase of immune responses. Recent evidence has demonstrated that lymphokines regulate monocyte cell surface expression of DR antigens and, consequently, the ability of monocytes to activate T lymphocytes in an antigen‐specific manner. In this report cloned interferons and a homogeneous cell line were used to demonstrate that interferon possesses these immunoregulatory functions. Cells of the promyelocytic cell line HL60, when incubated in vitro with recombinant gamma (IFN‐γ) and with alpha interferons (IFN‐α), expressed enhanced levels of DR antigen as determined by both cytotoxicity and fluorescence‐activated cell sorter analyses. Lower concentrations of IFN‐γ than IFN‐α were needed to induce DR expression, and a higher percentage of monocytes were induced to express DR antigen by IFN‐γ than IFN‐α. HL60 cells preincubated with lymphocyte‐derived lymphokines or IFN‐α also stimulated a significantly better in vitro allogeneic response in the mixed leukocyte reaction than untreated HL60 cells. Thus, interferon enhanced both the phenotypic expression of DR antigens of HL60 cells and their functional ability to inititiate T‐lymphocyte responses to an alloantigen.