Premium
Prostaglandin E 2 Receptors on Human Peripheral Blood Monocytes
Author(s) -
ERIKSEN E. F.,
RICHELSEN B.,
BECKNIELSEN H.,
MELSEN F.,
NIELSEN H. KRÆMMER,
MOSEKILDE L.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01416.x
Subject(s) - receptor , endocrinology , prostaglandin e , medicine , intracellular , monocyte , prostaglandin , prostaglandin e2 receptor , chemistry , cyclase , biology , biochemistry , agonist
The prostaglandin E 2 (PGE 2 ) receptor on human peripheral blood monocytes is characterized. The receptor binding at physiological temperature and pH was saturable, specific, and reversible. Scatchard analysis of binding data revealed a linear plot giving a K d = 1.1 ± 10 ‐9 mol/l and B max =4.1 fmol/10 7 cells, equal to 240 binding sites per cell. PGE 2 increased intracellular cyclic adenosine 5′‐monophosphate by a maximal factor of 3. PGF 2α and archidonic acid had no stimulatory effects on adenyl cyclase, in accordance with their low binding to the cells. The characterization of the PGE 2 receptor on human monocytes creates a basis for the study of the clinical significance of changes in PGE 2 ‐receptor binding in disease states involving PGE 2 ‐monocyte interactions such as various immunological disorders and bone resorption.