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Isopycnic Ultracentrifugation of Immune Complexes
Author(s) -
RøAHL E.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01407.x
Subject(s) - isopycnic , ultracentrifuge , chemistry , analytical ultracentrifugation , antibody , immune system , bovine serum albumin , immune complex , antigen antibody complex , antigen , biochemistry , centrifugation , microbiology and biotechnology , chromatography , biology , immunology
Isopycnic ultracentrifugation is frequently applied for preparative isolation of macromolecules. Using bovine serum albumin (BSA)‐anti‐BSA antibody complexes as a model system. isopycnic bunding of complexes was observed in CsCl. Nycodenz. and sucrose gradients. In CsCl gradients, free antigen or antibody could not be separated from the immune complexes. Variations in antigen to antibody ratio from equivalence and in the amount of complement present during complex formation resulted in zone broadening and banding at slightly lower densities in Nycodenz. This was not observed in sucrose. Serum immune complexes were isolated from patients with rheumatoid arthritis or ankylosing spondylitis. Banding of in vivo‐formed immune complexes was observed more frequently in sucrose than in Nycodenz.