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2‐Mercaptoethanol‐Dependent Lipopolysaccharide‐Responsive B Cells in Nylon‐Wool‐Fractionated Spleen Cell Preparations
Author(s) -
EASTMAN A. Y.,
LAWRENCE D. A.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01400.x
Subject(s) - lipopolysaccharide , splenocyte , spleen , stimulation , thymidine , microbiology and biotechnology , chemistry , cell growth , immunology , biology , andrology , biochemistry , endocrinology , medicine , dna
The ability of lipopolysaccharide (LPS) to influence lymphocyte mitogenresponsiveness to 2‐mercaptoethanol (2‐ME) was investigated. 2‐ME was mitogenic for splenocytes only when fetal bovine serum lots containing elevated endotoxin levels were used to supplement (5%) the culture medium. 2‐ME did not stimulate mitosis of nylon‐wool‐fractionated splenocytes (NWSC): however, 2‐ME induced significan proliferation in the NWSC cell preparations whenendotoxin‐deficient culture medium was supplemented with doses of LPS whichalone did not stimulate significant proliferation. The sIg* and Thy‐1 ‐ /Lyt‐l ‐ fractions of NWSC, which represented less than 5% of the cells in the‘T‐cell preparations', were almost completely dependent on 2‐ME for proliferation in response to LPS stimulation. These data indicate that nylon‐wool‐fractionated preparations cuntain splenic B cells that incorporate significant amounts of [ 3 H]thymidine when stimulated with LPS and 2‐ME.