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Establishment of a Long‐Term Cultured Suppressor T‐Cell Line Specific for Guanosine
Author(s) -
IMAI Y.,
NAIKI M.,
OSAWA T.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb01037.x
Subject(s) - guanosine , spleen , in vitro , microbiology and biotechnology , biology , mitomycin c , suppressor , cell culture , chemistry , immunology , andrology , medicine , biochemistry , genetics , gene
Induction of suppressor cells specific for gmmosine in C57BL/6 mice with syngeneic spleen cells coupled with guanosine was demonstrated. Spleen cells from mice that had been tolerized by the injection of guanosine‐coupled syngeneic spleen cells (G–SC) were repeatedly stimulated with mitomycin C‐treated G‐SC (MMC‐G‐SC) in vitro. These cells effectively suppressed the secondary in vitro response to guanosine–keyhote limpet haemocyanin (G–KLH) but did not suppress the response to sheep erythrocytes (SRBC). From these cell populations a long‐term‐cultured suppressor T‐cell line specific for guanosine was established by using interleukin‐2 and MMC‐G‐SC. This cell line suppressed the response to G‐KLH but did not suppress the response to trinitrophenyl–KLH and SRBC. The suppressive effect was completely eliminated by treatment with anti‐Thy 1.2 and complement