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Role of HLA Class II Products in Proliferative T‐Lymphocyte Responses to PPD
Author(s) -
EDEN W.,
ELFERINK B. G.,
HERMANS J.,
VRIES R. R. P.,
ROOD J.J.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb01032.x
Subject(s) - immunology , human leukocyte antigen , t cell , lymphocyte , t lymphocyte , monocyte , cell , antigen , biology , medicine , immune system , genetics
HLA class II determinants were analysed for their role in monoeyte‐T‐cell infractions in the prolifeniuve response to purified protein derivative (PPD). Allogeneic cell combinations of monocytes and T cells were tested with a range of suboptimal PPD concentrations. For each cell combination tested, a summary measure to characterize the antigen‐induced response curve was calculated by means of regression analysis of the response in the dose of PPD added, thus obviating a further need to correct for mixed lymphocyte reactivity. In 200 distinct monocyte‐T‐cell combinations. HLA‐DR appeared io be the major restricting clement. Additionally, a marginal influence of HLA‐MB or ‐MT matching was observed and no evidence of HLA‐SB‐restricted responses. Remarkably, the HLA‐MB1 specificity was significantly associated with low responsiveness in DR sharing monocyte‐T‐cell combinations, indicating a modulating role of MB1 in DR‐restricted T‐cell responses.