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Detection of Lactoferrin and Myeloperoxidase Release from Single Neutrophils by a Protein A Plaque Assay
Author(s) -
RICHTER J.,
OLOFSSON T.,
BRITTON S.,
OLSSON I.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb01012.x
Subject(s) - lactoferrin , myeloperoxidase , degranulation , phagocytosis , chemistry , azurophilic granule , lysis , antiserum , granulocyte , microbiology and biotechnology , biochemistry , inflammation , biology , immunology , antibody , receptor
A haemolytic plaque assay was adopted to detect release of lactoferrin and mycloperoxidase (MPO) from single neutrophils. Target erythrocytes coated with protein A were bound as a monolayer by poly‐ l ‐lysine to the surface of plastic dish. Secreted lactoferrin and MPO induced plaque formation dependent on the reaction with complement and specific antiserum producing lysis of the protein‐A‐coated sheep red blood cells Lactoferrin was found to be released spontaneously from a fraction of neutrophils while MPO was released only after phagocytosis, reflecting different mechanisms for degranulation of MPO‐containing azurophil and lactoferrin‐containing specific granules.