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The Effect of Monoclonal Antibodies against Escherichia coli Type 1 Pili and Capsular Polysaccharides on the Interaction between Bacteria and Human Granulocytes
Author(s) -
SÖDERSTRÖM T.,
ÖHMAN L.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb01006.x
Subject(s) - monoclonal antibody , pilus , microbiology and biotechnology , antibody opsonization , escherichia coli , antibody , bacteria , biology , phagocytosis , bacterial capsule , mannan , granulocyte , polysaccharide , opsonin , immunology , biochemistry , genetics , virulence , gene
Monoclonal antibodies against Escherichia coli type 1 pili and the K13 capsular polysaccharide strongly influenced the interaction between human polymorphonuclear leucocytes (PMNL) and E. coli 06:K13:H1. Bacteria with type 1 pili, associated with the neutrophils, caused a metabolic activation but were not ingested. Addition of monoclonal antibodies to type 1 pili resulted in increased association, but also phagocytosis and metabolic activation of the granulocytes. Monoclonal antibodies against the Kl3 polysaccharide strongly stimulated phagocytosis, especially of the type 1 piliated bacteria, suggesting a synergistic effect between binding of type 1 pili and the Fc part of the capsular antibodies to the PMNL. Addition of D‐mannose inhibited the opsonization of type 1 piliated E. coli 06:K13:H1 in the presence of both type 1 pilus antibodies and capsular antibodies. Monoclonal anti‐idiotypic (anti‐anti‐capsular) antibodies reduced the association with the PMNL of the bacteria preopsonized with anti‐capsular antibodies. The bacterial ingestion and the metabolic activation of the PMNL were also reduced, suggesting a role for anti‐idiotypic antibodies in specific modulation of inflammation.