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Effects of Interferon‐α/β and Interferon‐γ Preparations on Phagocytosis by Mouse Peritoneal Macrophages
Author(s) -
ROLLAG H.,
DEGRÉ M.,
SONNENFELD G.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00988.x
Subject(s) - opsonin , phagocytosis , ingestion , microbiology and biotechnology , receptor , antiserum , interferon , immunoglobulin g , biology , interferon gamma , fc receptor , immunology , antibody , chemistry , immune system , endocrinology , biochemistry
The influence of murine alpha/beta‐interferon (Mu IFN‐α/β) and murine gamma‐interferon (Mu IFN‐γ) preparations on the attachment and ingestion phase of phagocytosis by mouse peritoneal macrophages (MPM) was studied. A non‐opsonized strain of Escherichia coli , IgG‐opsonized E. coli , and sheep erythrocytes opsonized with IgG (E‐IgG) and IgM plus complement factor C3b (E‐IgMC) were used as test particles. Pretreatment of MPM with 10 2 ‐10 3 U/ml of Mu IFN‐α/β for 24 h enhanced both attachment and ingestion of bacteria or erythrocytes mediated by the non‐specific receptor, the Fc receptor, or the C3b receptor. Higher concentrations had no such effects. In contrast, treatment of MPM with 10 1 ‐10 2 U/ml of Mu IFN‐γ suppressed attachment and ingestion of non‐opsonized and IgG‐opsonized E. coli and of E‐IgG by 10–40%. Mu IFN‐γ did not influence attachment and ingestion of E‐IgMC. The effects were neutralized by specific anti‐IFN antiserum. The data indicate that the IFN effect on phagocytic activity is, at least to a large extent, due to modifications of the surface receptors.