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Lymphoproliferation Induced in Mouse Spleen Cells by Mycoplasma arthritidis Mitogen
Author(s) -
KIRCHNER H.,
GIEBLER D.,
KEYSSNER K.,
NICKLAS W.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00986.x
Subject(s) - spleen , microbiology and biotechnology , biology , macrophage , splenocyte , immunology , in vitro , biochemistry
Spleen cells of various mouse strains (e.g. BALB/c, C3H, and CBA) reacted towards MAS (a mitogen derived from supernatants of cultured Mycoplasma arthritidis ) with a marked lymphoproliferative response. This reactivity was T‐cell‐dependent. It was reduced by 90% after removal of macrophages by passage of the spleen cells through Sephadex G‐10 columns. Addition of 2‐mercaptoethanol (2‐ME) to macrophage‐depleted CBA spleen cells completely restored the response to MAS. Spleen cells of C57BL/6 and C57BL/10 mice were unreactive to MAS, even in the presence of macrophages, and this non‐reactivity was controlled by the I‐region of H‐2. Other mouse strains that, similarly to C57BL/6, lack the expression of I‐E on the cell surface (that is, mice of the haplotype H‐2f, H‐2q, and H‐2s) were also non‐responsive to MAS. However, the addition of 2‐ME to spleen cells of non‐responder mice resulted in high lymphoproliferative responses to MAS, which were as high as those of CBA spleen cells. The reaction of C57BL/6 spleen cells to MAS in the presence of 2‐ME again was T‐cell‐dependent, as shown by data with spleen cells of homozygous nude mice and spleen cells treated by anti‐thy‐1 and C. A macrophage dependency of this response was also evident. When C57BL/6 spleen cells were vigorously freed of accessory cells by the use of nylon wool columns, the MAS response could no longer be restored by 2‐ME.