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Preparative Isolation of Immune Complexes from Serum by Sucrose Gradient Ultracentrifugation
Author(s) -
RØDAHL E.,
IVERSEN O.J.,
DALEN A. B.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00973.x
Subject(s) - analytical ultracentrifugation , ultracentrifuge , ankylosing spondylitis , isopycnic , immune system , psoriasis , chemistry , antigen , centrifugation , immune complex , chromatography , antibody , density gradient , differential centrifugation , immunology , medicine , biochemistry , physics , quantum mechanics
Immune complexes were isolated by ultracentrifugation in sucrose gradients (20‐65% (w/w)). The centrifugation procedure was demonstrated 10 be isopycnic. The banding density of the complexes was influenced by the chemical nature and molecular size of the antigen and by the antigen to antibody ratio. The method was applied for preparative isolation of immune complexes from patients with systemic lupus erythematosus. rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, and uncomplicated psoriasis.