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Functional Analysis of B‐L (Ia‐Like) Antigen‐Bearing Chicken Peripheral Blood Cells
Author(s) -
HÁLA K.,
BOYD R. L.,
WOLF H.,
BÖCK G.,
WICK G.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00972.x
Subject(s) - phytohaemagglutinin , microbiology and biotechnology , in vitro , fluorescein isothiocyanate , concanavalin a , pokeweed mitogen , population , antigen , peripheral blood , in vivo , immunofluorescence , immunology , biology , chemistry , antibody , medicine , fluorescence , biochemistry , physics , environmental health , quantum mechanics
The function of B‐L (Ia‐equivalent)‐positive (B‐L + ) and ‐negative (B‐L ‐ ) chicken peripheral blood lymphocytes (PBL) was studied in vitro and in vivo. The PBL were first stained in direct immunofluorescence tests with a fluorescein isothiocyanate‐labelled anti‐B‐L alloantiserum and then separated by means of a fluorescence‐activated cell sorter. In agreement with our previous findings, B‐L ‐ cells showed functional properties of T lymphocytes, responding to concanavalin A and phytohaemagglutinin‐P in vitro and inducing a graft‐versus‐host (GVH) reaction when injected into allogeneic embryos. Sorted B‐L + gave no responses in any of these assays. Neither B‐L + nor B‐L ‐ cells, when tested alone, responded significantly to pokeweed mitogen, but mixtures of the two restored the responsiveness to that of the original unsorted suspension. Of the B‐L + PBL, 10% were T cells, which may account for the low GVH reactivity given by this population.