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Functional and Chemical Characterization of B‐Cell Growth Factor Produced by Normal Cloned T Helper Cells
Author(s) -
PETTERSSON S.,
LEANDERSSON T.,
FORSGREN S.,
POBOR G.,
COUTINHO A.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00971.x
Subject(s) - microbiology and biotechnology , biology , secretion , lipopolysaccharide , b cell , cell , gel electrophoresis , lymphokine , antibody , chemistry , antigen , immunology , biochemistry
Media conditioned by clones of normal helper T cells exposed to appropriate antigenpresenting cells contain growth‐promoting activity for B‐cell blasts induced either by lipopolysaccharide or on direct interaction with competent helper cells. This B‐cell growth factor (TH‐BGA pet ) is recovered on sodium dodecyl sulphate polyacrylamide gel electrophoresis corresponding to mol. wt of 15,000–20,000 displays no mitogenicity for small, non‐induced B lymphocytes and is completely devoid of the ability to activate immunoglobulin secretion in proliferating B cells. These results are ascribed to the activity derived from normal T cells, with the same characteristics as BSF‐pl previously obtained from lymphomas and hybridomas. Since hybridization of these T helper cells results in the constitutive production of BSF‐pl in the absence of macrophages, these experiments demonstrate that BSF‐pl is a normal T‐cell product.