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On the Molecular Basis of T Helper Cell Function
Author(s) -
RUBIN B.,
REININGER L.,
SUZAN M.,
FUERI J.,
DENIZOT F.,
LANDOLFO S.,
AMBLE J. G,
LONAI P.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00967.x
Subject(s) - b cell , microbiology and biotechnology , polyclonal b cell response , antigen , polyclonal antibodies , lymphokine , biology , cell culture , antibody , inducer , t cell , chemistry , immune system , immunology , biochemistry , gene , b cell receptor , genetics
B‐lymphocyte promotor factors (B‐LPF) are defined as T‐cell‐derived, released molecules that trigger polyclonal induction of B‐cell differentiation into antibody‐forming cells. B‐LPF activity is independent of antigen, and it apparently induces only IgM‐producing B cells. B‐LPF was discovered as products of an antigen‐specific. I‐A b ‐restricted T‐cell line. We here show that B‐LPF is produced also by lymphoma cells derived from this T‐cell line or by T‐cell hybrids constructed by fusing the T‐cell line with BW5147 thymoma cells. A chicken gamma globulin‐specific T‐cell hybridoma clone also produced B‐LPF. Biological assays demonstrated that B‐LPF‐containing supernatants did not contain IL‐1, IL‐2, B‐cell growth factor, or allogeneic effector factor. Biochemical studies showed that B‐LPF was precipitated by 50% (NH 4 ) 2 SO 4 saturation iind that at least three types of molecules were involved in B‐LPF activity: molecules with molecular weights of >90,000, 50,000–90,000 and 10,000–25,000.The relationship between B‐LPF and antigen‐specific helper/inducer factors is discussed.