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Plaque‐Forming Cells in Man
Author(s) -
TAURIS P.,
JOHNSEN H. E.,
CHRISTIANSEN S. E.,
KISSMEYERNIELSEN F.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00950.x
Subject(s) - antigen , microbiology and biotechnology , secretion , human leukocyte antigen , antibody , biology , t lymphocyte , immunology , peripheral blood , endocrinology
We have investigated the ability of allogeneic, irradiated T lymphocytes to induce proliferation and immunoglobulin (Ig) secretion in untreated peripheral blood B lymphocytes. Non‐mitogen‐activated co‐cultures of isolated T and B lymphocytes from selected, full‐house HLA‐A, B and D/DR antigen‐phenotyped donors were reconstituted in a ratio of 4:1. Proliferation was assessed on day 5–6 of culture by the 3 H‐thymidine incorporation technique, and the Ig secretion was monitored on day 6 with a protein A plaque‐forming cell (PFC) assay. B lymphocytes were able to diffferentiate into PFC, and the number of plaques was significantly higher in cultures of cells with two HLA‐D/DR antigen incompatibilities than in those sharing one antigen. In cultures of peripheral blood lymphocytes with no HLA‐D/DR antigen difference, only a few PFC developed. HLA‐A and B antigens had no influence on the response. Further, monocytes were not an absolute requirement for allogeneic activation of B cells. Sonicated T cells and culture supernatants from allogeneic T‐ and B‐cells cultures were not able to induce PFC formation in B lymphocytes. Our results indicate that the PFC response obtained in non‐mitogen‐activated cultures of allogeneic T and B lymphocytes is dependent on HLA‐D/DR disparity or on genes encoded in the HLA‐D/DR region.