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Surface Membrane Glycoproteins of Macrophage‐like Synovial Cells from Rheumatoid and Control Patients
Author(s) -
KOURI T.,
HEINO J.,
KRONELD U.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00942.x
Subject(s) - synovial membrane , macrophage , glycoprotein , rheumatoid arthritis , chemistry , synovial fluid , microbiology and biotechnology , peripheral blood mononuclear cell , pathology , percoll , immunology , biology , medicine , centrifugation , biochemistry , in vitro , osteoarthritis , alternative medicine
Synovial cells were prepared by enzyme digestion and Percoll gradient centrifugation of rheumatoid arthritis (RA) synovial specimens or by trypsin‐rinsing of non‐inflammatory cadaver joints. Most (70–80%) of the cells from RA patients were OKIa‐positive macrophage‐like cells, 10–20% other OKIa‐positive cells, and about 10% fibroblastic cells. whereas 90% of the normal synovial cells were OKIa‐positive macrophage‐like cells and the rest fibroblasts. These adherent synovial cells were compared with fibroblastic synovial cells obtained by sequential passaging of explained dividing cells. Periodate‐[ 3 H]borohydride labelling followed by SDS‐gradient gel electrophoresis demonstrated similar sialo‐glycoprotein patterns in both the adherent synovial cells and synovial fibroblasts. The molecular weights of the main surface glycoproteins resembled closely those of skin fibroblasts but not those of peripheral blood monocytes, RA samples showed inconsistent heterogeneity. The results indicate either that all synovial cells possess a similar basic structure or that macrophages of peripheral blood origin express fibroblastic contact glycoproteins when settling down into synovium.