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Depletion of Murine Anti‐Azobenzenearsonate Plaque‐Forming Cells by Derivatized Polyacrylamide Beads
Author(s) -
BALIAN G. P.,
BELLONE C. J.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00940.x
Subject(s) - polyacrylamide , chemistry , polyacrylamide gel electrophoresis , microbiology and biotechnology , biology , biochemistry , enzyme
The depletion of secondary p ‐azobenzenearsonate plaque‐forming cells (ABA‐PFC) by affinity columns substituted with ABA was dependent on the primary dose, times elapsed after priming, and the nature of the side‐arm on the columns. Thus, higher priming doses of ABA coupled to keyhole limpet haemocyanin (50–500 μg of ABA‐KLH in complete Freund's adjuvant (CFA)) favoured depletion of ABA‐PFC by ABA coupled to a 6‐aminocaproyltyrosine side‐arm (SAC‐TYR‐ABA beads), whereas ABA‐PFC from mice primed with 1 μg of ABA‐KLH in CFA were never depleted; this latter population were only depleted by ABA coupled to a histamine side‐arm (HIST‐ABA beads) 7 months after priming. These data are consistent with the notion that two specificities, HIST‐ABA and TYR‐ABA, appear to emerge independently, lower priming doses inducing the preferential appearance of the HIST‐ABA specificity.