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Occurrence of Protein SAA‐Like Material in Human Endothelial Cells in Culture and Smooth‐Muscle Cells in Vessel Walls
Author(s) -
BJERVE Ø.,
NATVIG J. B.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00932.x
Subject(s) - cytoplasm , collagenase , umbilical cord , protein filament , antiserum , anatomy , smooth muscle , biology , microbiology and biotechnology , primary culture , chemistry , cell culture , immunology , biochemistry , antibody , endocrinology , enzyme , genetics
In the present study human endothelial cells were isolated from the veins of umbilical cord by collagenase treatment and kept in primary culture. Antiserum with monospecific activity against protein SAA was used in an indirect immunotluorescence study. SAA‐Iike material was shown to he present in the cytoplasm of endothelial cells thai were grown on glass discs and formed a typical uniform monolayer. It was orientated in a cytoplasmic filament pattern. SAA‐like material was also demonstrated in the cytoplasm of smooth‐muscle cells in vessel walls in cryostat sections from placenta and umbilical cord.