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Autologous Mixed Lymphocyte Reaction in Man
Author(s) -
GUPTA S.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00918.x
Subject(s) - mixed lymphocyte reaction , monoclonal antibody , stimulation , immunology , in vitro , null cell , t lymphocyte , lymphocyte , lymphocyte subsets , t cell , andrology , antibody , microbiology and biotechnology , medicine , chemistry , cell culture , biology , immune system , biochemistry , genetics
Peripheral blood from 15 young (20–30 years) and 15 aging (65–80 years) subjects was analysed for the proliferative response of T cells upon stimulation with non‐T cells in the autologous mixed culture reaction (AMLR) and allogeneic MLR, and for the proportion of monoclonal antibody‐defined lymphocyte subsets and monocytes. No significant difference was observed in the AMLR or allogeneic MLR between T and non‐T cells in aging and young subjects. However, when non‐T cells were further fractionated into adherent monocytes and non‐adherent B cells (B cells and null cells), the AMLR between macrophages and T cells was significantly ( P < 0.05) higher in young subjects than in simultaneously studied aging subjects. In contrast, the AMLR between T cells and B cells was significantly ( P < 0.05) higher in the aging subjects than in the young group. In the T‐T AMLR (using phytohaemagglutin‐stimulated T cells as stimulators), aging subjects had a significantly ( P < 0.05) lower proliferative response than simultaneously studied young subjects. In vitro addition of purified interleukin 2 reconstituted the T‐T AMLR to the base‐line T‐T AMLR in young humans. No significant difference was observed in the allogeneic MLR and lymphocyte subsets between the two groups. The significance of these observations is discussed.